Special-deal PhiC31 Transformation Packages

(Please read below, service terms included)

Plan K - PhiC31 non-Standard service - PhiC31 one-shoot-3x-Plan H-Service for large BACs of >30 kb only and difficult-to-transform plasmids (CNY2940): Similar idea of 1.5x price of Plan H, we provide one-shoot-3x-Plan H service, i.e., inject 600+ embryos at once, no free re-injection and no store credits if no transformant. You may choose 1, 2 or 3 strains for the 600+ embryos. Free Service W1 (PCR confirmation for up to 6 lines) is included if there is transformants. Recommended for testing different landing sites for large BACs and difficult-to-transform plasmids. If you wish the resulting lines be balanced, the charge is ￥74/line (please put this request on the remark); if you wish to receive the regular Plan K shipment plus a later balanced lines shipment, the extra charge is ￥105 + ￥74/line and the shipping fee.

Plan M - MiMIC Injection Service Package #1 (CNY3890): This package includes the below 3 items. The original price was ￥5080, discounted to ￥3890.

1. New strain ordering-expanding-preparing (CNY740 per strain)
This includes the direct BDSC ordering fee (non-refundable if you send us your strain). We order the strain from the BDSC, expand and prepare it for injection. It's recommended to order the Plan N-item#4 (Service W3) below to double check the strain before the injection. We keep the strain for 3 months after the completion of each Plan M.

2. DNA Preparation Service Z1 (CNY190)
This Plan M package includes the free DNA Preparation Service Z1 (non-refundable if you request to use your DNA directly).

3. Non-standard Plan I - Injection-screening-balancing (CNY4150)

- setup the injection cage by crossing the MiMIC strain to proper strain with PhiC31 integrase source, collect F1 embryos.

- inject 300+ embryos (i.e. 1.5x quantity of regular Plan H).

- setup the G0 crosses using proper balancer stocks.

- screen for "loss of yellow" or "gain of XX" phenotype. We screen up to 8 individual lines.

- balance the G1 founder using proper balancer stocks

When using this service, we will try to get at least 2 independent transformants for a reasonably sized construct (smaller than 13 kb) due to the fact that the MiMIC integration event is orientational. We will send you maximum 8 lines even if there are more found in the screening process. For constructs smaller than 13 kb, we may offer a free 2nd injection if we do not get 2 or more lines from the 1st injection; we may offer a 3rd injection (we will charge you full amount regardless of the result) or ￥840 store credit if we do not get transformant from the first two round of injections. For constructs larger than 13 kb, we may offer a free 2nd injection if we do not get 2 or more lines from the 1st injection; we will charge you full amount regardless of the result of the first two round of transformations.

In the case of no injection after we prepare the strain, we only charge ￥740 if we never perform the injection work.

In the case of one strain multiple constructs injection, we may return ￥525 per additional constructs for duplicated strain charge. Please contact us in this case. e.g. if you place 3 Plan M orders using the same strain, i.e. ￥3890x3-￥525x2=￥10620.

Plan N - MiMIC Injection Service Package #2 (CNY4830): This package includes the above Plan M and the below 2 items. The original price was ￥6520, discounted to ￥4830.

4. Service W3 - for Plan M, PCR confirmation of the attP sites for the new MiMIC strain, construct and construct/strain background (CNY640 includes 1 strain and 1 construct, CNY480 per additional construct). We design the primers, order the oligos, perform genomic DNA extractions and perform the PCR works for you. This is very important to verify the strain and the construct before/for the long Plan M/N progress.

5. Service W4 - Orientation screening for Plan M lines (CNY320 + CNY160 per additional line). Must order "Service W3" if you order "Service W4". By default, we only test Four lines if we get 4 or more lines. i.e. the minimum charge is ￥280 if we only get one line. e.g. If we get 8 lines we only test 4 lines, i.e. ￥320+￥160+￥160+￥160=￥800. If you want to test more, please email us.